├── Images
├── R-UNet.png
├── Results1.png
├── Results2.png
└── Results3.png
├── LICENSE.txt
├── README.md
├── Step1_PreProcessing.py
├── Step2_ContourExtraction.py
├── TestFoldAndWeights.zip.001
├── TestFoldAndWeights.zip.002
├── TestFoldAndWeights.zip.003
├── TestFoldAndWeights.zip.004
├── TestFoldAndWeights.zip.005
├── TestFoldAndWeights.zip.006
├── TestFoldAndWeights.zip.007
├── data_loader.py
├── lib
└── FindFiles.py
├── model.py
├── predict.py
└── train.py
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672 | the library. If this is what you want to do, use the GNU Lesser General
673 | Public License instead of this License. But first, please read
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--------------------------------------------------------------------------------
/README.md:
--------------------------------------------------------------------------------
1 | # 3D RU-Net
2 |
3 | Code for the paper entitled "3D RoI-aware U-Net for Accurate and Efficient Colorectal Cancer Segmentation"(https://arxiv.org/abs/1806.10342).
4 |
5 | The latest codes along with weights and a test fold are now released.
6 |
7 | Tips: a recent attempt that transfers training and inferencing to fp16 data format can further enlarge applicable volume sizes.
8 |
9 | 
10 |
11 | Here are some results of colorectal cancer segmentation, which is the case of the paper; and illustrations of another task, mandible and masseter segmentation, showing the scalability of the proposed method.
12 |
13 | 
14 | 
15 |
16 | Latest experiment: simultaneously segmenting 14 organs from pelvic CTs in ~0.5s (We trained this model with 24 training samples).
17 |
18 | 
19 |
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/Step1_PreProcessing.py:
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1 | # -*- coding: utf-8 -*-
2 | """
3 | @author: HuangyjSJTU
4 | """
5 | import SimpleITK as sitk
6 | import numpy as np
7 | import sys
8 | import os
9 | sys.path.append('./lib/')
10 | import matplotlib.pyplot as pl
11 | from PIL import Image as Img
12 | from FindFiles import findfiles
13 | import dicom
14 | import cv2
15 | from skimage import filters
16 | from skimage.measure import label,regionprops
17 | #For intensity normalization
18 |
19 | DataRoot='../Data/send/'
20 | ModelName='/t2-fov/'
21 | ManualNormalize=True
22 | ResRate=['HighRes','MidRes','LowRes']
23 | ToSpacing={'HighRes':[1,1,4],'MidRes':[1.5,1.5,4],'LowRes':[2,2,4]}
24 |
25 | def ReadImageAndLabel(CasePath,inverted=False):
26 | #Reading Images
27 | Reader = sitk.ImageSeriesReader()
28 | name=findfiles(CasePath+'img/','*.dcm')
29 | for i in range(len(name)):
30 | name[i]=int(name[i][0:-4])
31 | name=sorted(name)
32 | name=name[::-1]
33 | for i in range(len(name)):
34 | #print name[i],'\n'
35 | name[i]=CasePath+'img/'+str(name[i])+'.dcm'
36 |
37 | Reader.SetFileNames(name)
38 | Image = Reader.Execute()
39 | Spacing=Image.GetSpacing()
40 | Origin = Image.GetOrigin()
41 | Direction = Image.GetDirection()
42 |
43 |
44 |
45 | #Reading Labels
46 | name=findfiles(CasePath+'label/','*.PNG')
47 | name=sorted(name)
48 | for i in range(len(name)):
49 | name[i]=CasePath+'label/'+name[i]
50 | #print name
51 | #Sometimes labels are inverted along Z axis and should be rectified in this dataset
52 | if inverted:
53 | pass
54 | else:
55 | name=name[::-1]
56 | # for i in range(len(name)):
57 | # print name[i]+'\n'
58 | Reader.SetFileNames(name)
59 | Label = Reader.Execute()
60 | LabelArray=sitk.GetArrayFromImage(Label)
61 | LabelArray=((255-LabelArray[:,:,:,1])).astype(np.uint8)/255
62 | Label=sitk.GetImageFromArray(LabelArray)
63 | Label.SetSpacing(Spacing)
64 | Label.SetOrigin(Origin)
65 | Label.SetDirection(Direction)
66 | return Image,Label
67 |
68 | def Resampling(Image,Label):
69 | Size=Image.GetSize()
70 | Spacing=Image.GetSpacing()
71 | Origin = Image.GetOrigin()
72 | Direction = Image.GetDirection()
73 | ImagePyramid=[]
74 | LabelPyramid=[]
75 | for i in range(3):
76 | NewSpacing = ToSpacing[ResRate[i]]
77 | NewSize=[int(Size[0]*Spacing[0]/NewSpacing[0]),int(Size[1]*Spacing[1]/NewSpacing[1]),int(Size[2]*Spacing[2]/NewSpacing[2])]
78 | Resample = sitk.ResampleImageFilter()
79 | Resample.SetOutputDirection(Direction)
80 | Resample.SetOutputOrigin(Origin)
81 | Resample.SetSize(NewSize)
82 | Resample.SetInterpolator(sitk.sitkLinear)
83 | Resample.SetOutputSpacing(NewSpacing)
84 | NewImage = Resample.Execute(Image)
85 | ImagePyramid.append(NewImage)
86 |
87 | Resample = sitk.ResampleImageFilter()
88 | Resample.SetOutputDirection(Direction)
89 | Resample.SetOutputOrigin(Origin)
90 | Resample.SetSize(NewSize)
91 | Resample.SetOutputSpacing(NewSpacing)
92 | Resample.SetInterpolator(sitk.sitkNearestNeighbor)
93 | NewLabel = Resample.Execute(Label)
94 | LabelPyramid.append(NewLabel)
95 | return ImagePyramid,LabelPyramid
96 |
97 | #We shift the mean value to enhance the darker side
98 | UpperBound=1.0
99 | LowerBound=-4.0
100 |
101 | def Normalization(Image):
102 | Spacing=Image.GetSpacing()
103 | Origin = Image.GetOrigin()
104 | Direction = Image.GetDirection()
105 | Array=sitk.GetArrayFromImage(Image)
106 | Array_new=Array.copy()
107 | Array_new+=np.min(Array_new)
108 | Array_new=Array_new[Array_new.shape[0]/2-5:Array_new.shape[0]/2+5]
109 | Mask=Array_new.copy()
110 | for i in range(Array_new.shape[0]):
111 | otsu=filters.threshold_otsu(Array_new[i])
112 | Mask[i][Array_new[i]<0.5*otsu]=0
113 | Mask[i][Array_new[i]>=0.5*otsu]=1
114 | MaskSave=sitk.GetImageFromArray(Mask)
115 | MaskSave=sitk.BinaryDilate(MaskSave,10)
116 | MaskSave=sitk.BinaryErode(MaskSave,10)
117 | Mask=sitk.GetArrayFromImage(MaskSave)
118 |
119 | Avg=np.average(Array[Array_new.shape[0]/2-5:Array_new.shape[0]/2+5],weights=Mask)
120 | Std=np.sqrt(np.average(abs(Array[Array_new.shape[0]/2-5:Array_new.shape[0]/2+5] - Avg)**2,weights=Mask))
121 | Array=(Array.astype(np.float32)-Avg)/Std
122 | Array[Array>UpperBound]=UpperBound
123 | Array[Array0):
38 | LabelErode=Label[z]-cv2.erode(Label[z],kernel)
39 | Contour[z]=LabelErode
40 | Contour=sitk.GetImageFromArray(Contour)
41 | Contour.SetOrigin(Origin)
42 | Contour.SetSpacing(Spacing)
43 | Contour.SetDirection(Direction)
44 | sitk.WriteImage(Contour,Root+Patient+'/'+ResRate+'/'+'Contour.mhd')
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/data_loader.py:
--------------------------------------------------------------------------------
1 | #!/usr/bin/env python3
2 | # -*- coding: utf-8 -*-
3 | """
4 | Created on Sat Feb 16 21:27:54 2019
5 |
6 | @author: customer
7 | """
8 | import numpy as np
9 | import random
10 | import SimpleITK as sitk
11 | from skimage.measure import label,regionprops
12 | from skimage import filters
13 | import torch
14 | #Maximum Bbox Cropping to Reduce Image Dimension
15 | def MaxBodyBox(input):
16 | Otsu=filters.threshold_otsu(input[input.shape[0]//2])
17 | Seg=np.zeros(input.shape)
18 | Seg[input>=Otsu]=255
19 | Seg=Seg.astype(np.int)
20 | ConnectMap=label(Seg, connectivity= 2)
21 | Props = regionprops(ConnectMap)
22 | Area=np.zeros([len(Props)])
23 | Area=[]
24 | Bbox=[]
25 | for j in range(len(Props)):
26 | Area.append(Props[j]['area'])
27 | Bbox.append(Props[j]['bbox'])
28 | Area=np.array(Area)
29 | Bbox=np.array(Bbox)
30 | argsort=np.argsort(Area)
31 | Area=Area[argsort]
32 | Bbox=Bbox[argsort]
33 | Area=Area[::-1]
34 | Bbox=Bbox[::-1,:]
35 | MaximumBbox=Bbox[0]
36 | return Otsu,MaximumBbox
37 |
38 | def DataLoader(Patient,opt,Subset='Train'):
39 | assert Subset in ['Train','Valid','Test']
40 | #Image Loading
41 | ImageInput=sitk.ReadImage(opt.DATA_ROOT+'/'+Subset+'/'+Patient+'/HighRes/'+'Image_2.mhd')
42 | ImageInput=sitk.GetArrayFromImage(ImageInput)
43 | RegionLabel=sitk.ReadImage(opt.DATA_ROOT+'/'+Subset+'/'+Patient+'/HighRes/'+'Label.mhd')
44 | RegionLabel=sitk.GetArrayFromImage(RegionLabel)
45 | ContourLabel=sitk.ReadImage(opt.DATA_ROOT+'/'+Subset+'/'+Patient+'/HighRes/'+'Contour.mhd')
46 | ContourLabel=sitk.GetArrayFromImage(ContourLabel)
47 | #Orig Shape Backup
48 | Shape=ImageInput.shape
49 | #Body Bbox Compute
50 | Otsu,MaximumBbox=MaxBodyBox(ImageInput)
51 |
52 |
53 | #Apply BodyBbox Cropping
54 | ImageInput=ImageInput[MaximumBbox[0]:MaximumBbox[3],MaximumBbox[1]:MaximumBbox[4],MaximumBbox[2]:MaximumBbox[5]]
55 | RegionLabel=RegionLabel[MaximumBbox[0]:MaximumBbox[3],MaximumBbox[1]:MaximumBbox[4],MaximumBbox[2]:MaximumBbox[5]]
56 | ContourLabel=ContourLabel[MaximumBbox[0]:MaximumBbox[3],MaximumBbox[1]:MaximumBbox[4],MaximumBbox[2]:MaximumBbox[5]]
57 |
58 | if Subset=='Train':
59 | Xinvert=random.randint(0,1)
60 | IntensityScale=random.uniform(0.9,1.1)
61 | else:
62 | Xinvert=False
63 | IntensityScale=1
64 | #Apply Intensity Jitterring
65 | ImageInput=((ImageInput-128.0)*IntensityScale+128.0)/255
66 | ImageInput[ImageInput>1]=1
67 | ImageInput[ImageInput<0]=0
68 | #Apply Random Flipping
69 | if Xinvert:
70 | ImageInput=ImageInput[:,:,::-1].copy()
71 | RegionLabel=RegionLabel[:,:,::-1].copy()
72 | ContourLabel=ContourLabel[:,:,::-1].copy()
73 |
74 | #To Tensor
75 | ImageTensor=np.zeros([1,1,ImageInput.shape[0],ImageInput.shape[1],ImageInput.shape[2]])
76 | ImageTensor[0,0]=ImageInput
77 | ImageTensor=ImageTensor.astype(np.float)
78 | ImageTensor=torch.from_numpy(ImageTensor)
79 | ImageTensor=ImageTensor.float()
80 | ImageTensor = ImageTensor.to(device=opt.GPU)
81 |
82 | RegionLabelTensor=np.zeros([1,2,ImageInput.shape[0],ImageInput.shape[1],ImageInput.shape[2]])
83 | RegionLabelTensor[0,1]=RegionLabel
84 | RegionLabelTensor[0,0]=1-RegionLabel
85 | RegionLabelTensor=torch.from_numpy(RegionLabelTensor)
86 | RegionLabelTensor=RegionLabelTensor.float()
87 | RegionLabelTensor=RegionLabelTensor.to(device=opt.GPU)
88 |
89 | ContourLabelTensor=np.zeros([1,2,ImageInput.shape[0],ImageInput.shape[1],ImageInput.shape[2]])
90 | ContourLabelTensor[0,1]=ContourLabel
91 | ContourLabelTensor[0,0]=1-ContourLabel
92 | ContourLabelTensor=torch.from_numpy(ContourLabelTensor)
93 | ContourLabelTensor=ContourLabelTensor.float()
94 | ContourLabelTensor=ContourLabelTensor.to(device=opt.GPU)
95 |
96 |
97 | return ImageTensor,RegionLabelTensor,ContourLabelTensor,Shape,MaximumBbox
98 |
99 | def ArbitraryDataLoader(Patient,opt,Subset='Test'):
100 | #Image Loading
101 | ImageInput=sitk.ReadImage(opt.DATA_ROOT+'/'+Subset+'/'+Patient+'/HighRes/'+'Image_2.mhd')
102 | ImageInput=sitk.GetArrayFromImage(ImageInput)/255.0
103 | #Orig Shape Backup
104 | Shape=ImageInput.shape
105 | #Body Bbox Compute
106 | Otsu,MaximumBbox=MaxBodyBox(ImageInput)
107 |
108 |
109 | #Apply BodyBbox Cropping
110 | ImageInput=ImageInput[MaximumBbox[0]:MaximumBbox[3],MaximumBbox[1]:MaximumBbox[4],MaximumBbox[2]:MaximumBbox[5]]
111 |
112 | #To Tensor
113 | ImageTensor=np.zeros([1,1,ImageInput.shape[0],ImageInput.shape[1],ImageInput.shape[2]])
114 | ImageTensor[0,0]=ImageInput
115 | ImageTensor=ImageTensor.astype(np.float)
116 | ImageTensor=torch.from_numpy(ImageTensor)
117 | ImageTensor=ImageTensor.float()
118 | ImageTensor = ImageTensor.to(device=opt.GPU)
119 |
120 |
121 |
122 |
123 | return ImageTensor,Shape,MaximumBbox
--------------------------------------------------------------------------------
/lib/FindFiles.py:
--------------------------------------------------------------------------------
1 | # -*- coding: utf-8 -*-
2 | """
3 | Created on Tue Aug 08 09:56:29 2017
4 |
5 | @author: Administrator
6 | """
7 | import os
8 | import glob
9 |
10 | def findfiles(dirname,pattern):
11 | cwd = os.getcwd() #保存当前工作目录
12 | if dirname:
13 | os.chdir(dirname)
14 |
15 | result = []
16 | for filename in glob.iglob(pattern): #此处可以用glob.glob(pattern) 返回所有结果
17 | result.append(filename)
18 | #恢复工作目录
19 | os.chdir(cwd)
20 | return result
--------------------------------------------------------------------------------
/model.py:
--------------------------------------------------------------------------------
1 | #!/usr/bin/env python3
2 | # -*- coding: utf-8 -*-
3 | """
4 | Created on Sat Feb 16 20:58:07 2019
5 |
6 | @author: customer
7 | """
8 |
9 | import numpy as np
10 | import torch
11 | import torch.nn as nn
12 | import torch.nn.functional as F
13 | import time
14 | from skimage.measure import label,regionprops
15 |
16 | class ResBlock(nn.Module):
17 | '''(conv => BN => ReLU) * 2'''
18 | def __init__(self, in_ch, out_ch, kernel, Inplace=True,Dilation=1):
19 | super(ResBlock, self).__init__()
20 | padding=((kernel[0]-1)//2,Dilation,Dilation)
21 | dilation=(1,Dilation,Dilation)
22 | self.Conv1=nn.Conv3d(in_ch, out_ch, kernel, padding=padding,dilation=dilation)
23 | self.BN1=torch.nn.InstanceNorm3d(out_ch)
24 | self.Relu=nn.ReLU(inplace=Inplace)
25 | self.Conv2=nn.Conv3d(out_ch, out_ch, kernel, padding=padding,dilation=dilation)
26 | self.BN2=torch.nn.InstanceNorm3d(out_ch)
27 | self.Conv3=nn.Conv3d(out_ch, out_ch, kernel, padding=padding,dilation=dilation)
28 | self.BN3=torch.nn.InstanceNorm3d(out_ch)
29 | def forward(self, x):
30 | x1 = self.Conv1(x)
31 | x2 = self.BN1(x1)
32 | x3 = self.Relu(x2)
33 | x4 = self.Conv2(x3)
34 | x5 = self.BN2(x4)
35 | x6 = self.Relu(x5)
36 | x7 = self.Conv3(x6)
37 | x8 = self.BN3(x7)
38 | x9 = torch.add(x8,x1)
39 | x10 = self.Relu(x9)
40 | return x10
41 |
42 |
43 | class inconv(nn.Module):
44 | def __init__(self, in_ch, out_ch, Inplace, Dilation=1):
45 | super(inconv, self).__init__()
46 | self.conv = ResBlock(in_ch, out_ch, (1,3,3), Inplace,Dilation)
47 | def forward(self, x):
48 | x = self.conv(x)
49 | return x
50 |
51 |
52 | class down(nn.Module):
53 | def __init__(self, in_ch, out_ch, p_kernel, Inplace,Dilation=1):
54 | super(down, self).__init__()
55 | self.mpconv = nn.Sequential(
56 | nn.MaxPool3d(p_kernel),
57 | ResBlock(in_ch, out_ch, (3,3,3), Inplace,Dilation)
58 | )
59 |
60 | def forward(self, x):
61 | x = self.mpconv(x)
62 | return x
63 |
64 |
65 | class up(nn.Module):
66 | def __init__(self, in_ch, out_ch, p_kernel, c_kernel, Inplace=True,learn=False,Dilation=1):
67 | super(up, self).__init__()
68 | self.p_kernel=p_kernel
69 | self.learn=learn
70 | if self.learn:
71 | self.up = nn.ConvTranspose3d(in_ch, out_ch, 2, stride=2)#torch.upsample(in_ch, out_ch,)#nn.ConvTranspose3d(in_ch, out_ch, 2, stride=2)
72 | self.fuse = ResBlock(in_ch, out_ch, c_kernel, Inplace,Dilation)
73 | self.conv = nn.Conv3d(in_ch, out_ch, (1,1,1))
74 | self.Relu=nn.ReLU(inplace=Inplace)
75 | def forward(self, x1, x2):
76 | if not self.learn:
77 | x1 = F.upsample(x1, size=(x1.size()[2]*self.p_kernel[0],x1.size()[3]*self.p_kernel[1],x1.size()[4]*self.p_kernel[2]),mode='trilinear')
78 | x1 = self.conv(x1)
79 | x1 = self.Relu(x1)
80 | x = torch.cat([x2, x1], dim=1)
81 | x = self.fuse(x)
82 | return x
83 |
84 | class OutconvG(nn.Module):
85 | def __init__(self, in_ch, out_ch):
86 | super(OutconvG, self).__init__()
87 | self.conv = nn.Conv3d(in_ch, out_ch, 1)
88 |
89 | def forward(self, x):
90 | x = self.conv(x)
91 | return x
92 | class OutconvR(nn.Module):
93 | def __init__(self, in_ch, out_ch):
94 | super(OutconvR, self).__init__()
95 | self.conv = nn.Conv3d(in_ch, out_ch, 1)
96 |
97 | def forward(self, x):
98 | x = self.conv(x)
99 | return x
100 |
101 | class OutconvC(nn.Module):
102 | def __init__(self, in_ch, out_ch):
103 | super(OutconvC, self).__init__()
104 | self.conv = nn.Conv3d(in_ch, out_ch, 1)
105 |
106 | def forward(self, x):
107 | x = self.conv(x)
108 | return x
109 |
110 | class GlobalImageEncoder(nn.Module):
111 | def __init__(self, opt):
112 | super(GlobalImageEncoder, self).__init__()
113 | self.opt=opt
114 | self.n_classes=len(opt.DICT_CLASS.keys())
115 | self.Inplace=True
116 | self.Base=opt.BASE_CHANNELS
117 | self.inc = inconv(1, self.Base,self.Inplace,Dilation=opt.STAGE_DILATION[0])
118 | self.down1 = down(self.Base, self.Base*2,(1,2,2),self.Inplace,Dilation=opt.STAGE_DILATION[1])
119 | self.down2 = down(self.Base*2, self.Base*4, (2,2,2),self.Inplace,Dilation=opt.STAGE_DILATION[2])
120 | self.LocTop = OutconvG(self.Base*4, self.n_classes)
121 | def forward(self,x):
122 | x1 = self.inc(x)
123 | x2 = self.down1(x1)
124 | x3 = self.down2(x2)
125 | LocOut=self.LocTop(x3)
126 | LocOut=F.softmax(LocOut)
127 | return LocOut,[x1,x2,x3]
128 | def TrainForward(self,x,y,GetGlobalFeat=False):
129 | y= F.max_pool3d(y,kernel_size=(2,4,4),stride=(2,4,4))
130 | LocOut,GlobalFeatPyramid=self.forward(x)
131 | if GetGlobalFeat:
132 | return LocOut,y,GlobalFeatPyramid
133 | else:
134 | return LocOut,y
135 | class LocalRegionDecoder(nn.Module):
136 | def __init__(self, opt):
137 | super(LocalRegionDecoder, self).__init__()
138 | self.opt=opt
139 | self.n_classes=len(opt.DICT_CLASS.keys())
140 | self.Inplace=True
141 | self.Base=opt.BASE_CHANNELS
142 | self.up1 = up(self.Base*4, self.Base*2,(2,2,2),(3,3,3),self.Inplace,False,Dilation=opt.STAGE_DILATION[1])
143 | self.up2 = up(self.Base*2, self.Base,(1,2,2),(1,3,3),self.Inplace,False,Dilation=opt.STAGE_DILATION[0])
144 | self.SegTop1 = OutconvR(self.Base, self.n_classes)
145 | self.SegTop2 = OutconvC(self.Base, self.n_classes)
146 | def forward(self,GlobalFeatPyramid,RoIs):
147 | x1=GlobalFeatPyramid[0]
148 | x2=GlobalFeatPyramid[1]
149 | x3=GlobalFeatPyramid[2]
150 | P_Region=[]
151 | P_Contour=[]
152 |
153 | for i in range(len(RoIs)):
154 | Zstart=RoIs[i][0]
155 | Ystart=RoIs[i][1]
156 | Xstart=RoIs[i][2]
157 | Zend=RoIs[i][3]
158 | Yend=RoIs[i][4]
159 | Xend=RoIs[i][5]
160 | #RoI TensorPyramid
161 | RoiTensorPyramid=[x3[:,:,Zstart:Zend,Ystart:Yend,Xstart:Xend],\
162 | x2[:,:,Zstart*2:Zend*2,Ystart*2:Yend*2,Xstart*2:Xend*2],\
163 | x1[:,:,Zstart*2:Zend*2,Ystart*4:Yend*4,Xstart*4:Xend*4]]
164 |
165 | p = self.up1(RoiTensorPyramid[0], RoiTensorPyramid[1])
166 | p = self.up2(p, RoiTensorPyramid[2])
167 | p_r = self.SegTop1(p)
168 | p_r = F.softmax(p_r)
169 |
170 | p_c = self.SegTop2(p)
171 | p_c = F.softmax(p_c)
172 |
173 | P_Region.append(p_r)
174 | P_Contour.append(p_c)
175 | return P_Region,P_Contour
176 | def TrainForward(self,GlobalFeatPyramid,RoIs,y_region,y_contour):
177 | Y_Region=[]
178 | Y_Contour=[]
179 | #Extract in-region labels
180 | for i in range(len(RoIs)):
181 | Zstart=RoIs[i][0]
182 | Ystart=RoIs[i][1]
183 | Xstart=RoIs[i][2]
184 | Zend=RoIs[i][3]
185 | Yend=RoIs[i][4]
186 | Xend=RoIs[i][5]
187 | y_region_RoI=y_region[:,:,Zstart*2:Zend*2,Ystart*4:Yend*4,Xstart*4:Xend*4]
188 | y_contour_RoI=y_contour[:,:,Zstart*2:Zend*2,Ystart*4:Yend*4,Xstart*4:Xend*4]
189 | Y_Region.append(y_region_RoI)
190 | Y_Contour.append(y_contour_RoI)
191 | P_Region,P_Contour=self.forward(GlobalFeatPyramid,RoIs)
192 | return P_Region,P_Contour,Y_Region,Y_Contour
193 |
194 | class RU_Net(nn.Module):
195 | def __init__(self, opt):
196 | super(RU_Net, self).__init__()
197 | self.opt=opt
198 | self.n_classes=len(opt.DICT_CLASS.keys())
199 | self.Inplace=True
200 | self.Base=48
201 | self.GlobalImageEncoder=GlobalImageEncoder(opt)
202 | self.LocalRegionDecoder=LocalRegionDecoder(opt)
203 | def forward_RoI_Loc(self, x,y):
204 | LocOut,Y=self.GlobalImageEncoder.TrainForward(x,y,False)
205 | return [LocOut,Y]
206 | def Localization(self,LocOut,Train=True):
207 | if Train:
208 | MAX_ROIS=self.opt.MAX_ROIS_TRAIN
209 | else:
210 | MAX_ROIS=self.opt.MAX_ROIS_TEST
211 | LocOut = LocOut.to(device='cpu')
212 | LocOut = LocOut.detach().numpy()
213 | RoIs=[]
214 | #num=0
215 | for i in range(1,self.n_classes):
216 | Heatmap = LocOut[0,i]
217 | Heatmap = (Heatmap-np.min(Heatmap))/(np.max(Heatmap)-np.min(Heatmap))
218 | Heatmap[Heatmap<0.5]=0
219 | Heatmap[Heatmap>=0.5]=1
220 | Heatmap*=255
221 | ConnectMap=label(Heatmap, connectivity= 2)
222 | Props = regionprops(ConnectMap)
223 | Area=np.zeros([len(Props)])
224 | Area=[]
225 | Bbox=[]
226 | for j in range(len(Props)):
227 | Area.append(Props[j]['area'])
228 | Bbox.append(list(Props[j]['bbox']))
229 | OverDesignRange=[1,2,2]
230 | for k in range(3):
231 | if Bbox[j][k]-OverDesignRange[k]<0:
232 | Bbox[j][k]=0
233 | else:
234 | Bbox[j][k]-=OverDesignRange[k]
235 | for k in range(3,6):
236 | if Bbox[j][k]+OverDesignRange[k-3]>=Heatmap.shape[k-3]-1:
237 | Bbox[j][k]=Heatmap.shape[k-3]-1
238 | else:
239 | Bbox[j][k]+=OverDesignRange[k-3]
240 | Area=np.array(Area)
241 | Bbox=np.array(Bbox)
242 | argsort=np.argsort(Area)
243 | Area=Area[argsort]
244 | Bbox=Bbox[argsort]
245 | Area=Area[::-1]
246 | Bbox=Bbox[::-1,:]
247 |
248 | max_boxes=MAX_ROIS[self.opt.DICT_CLASS[i]]
249 | if Area.shape[0]>=max_boxes:
250 | OutBbox=Bbox[:max_boxes,:]
251 | elif Area.shape[0]==0:
252 | OutBbox=np.zeros([1,6],dtype=np.int)
253 | OutBbox[0]=[0,0,0,1,1,1]
254 | else:
255 | OutBbox=Bbox
256 | for j in range(OutBbox.shape[0]):
257 | RoIs.append(OutBbox[j,:])
258 |
259 | return RoIs
260 |
261 |
262 | def TrainForward(self, x, y_region, y_contour):
263 | LocOut,y_region_down,GlobalFeatPyramid=self.GlobalImageEncoder.TrainForward(x,y_region,True)
264 | RoIs=self.Localization(LocOut,Train=True)
265 | P_Region,P_Contour,Y_Region,Y_Contour=self.LocalRegionDecoder.TrainForward(GlobalFeatPyramid,RoIs,y_region,y_contour)
266 |
267 |
268 | return P_Region,P_Contour,Y_Region,Y_Contour,RoIs,[LocOut,y_region_down]
269 | def forward(self, x):
270 | LocOut,GlobalFeatPyramid=self.GlobalImageEncoder.forward(x)
271 | RoIs=self.Localization(LocOut,Train=False)
272 | P_Region,P_Contour=self.LocalRegionDecoder(GlobalFeatPyramid,RoIs)
273 | return P_Region,P_Contour,RoIs
--------------------------------------------------------------------------------
/predict.py:
--------------------------------------------------------------------------------
1 | #!/usr/bin/env python2
2 | # -*- coding: utf-8 -*-
3 | """
4 | Created on Thu Jun 28 13:03:37 2018
5 |
6 | @author: customer
7 | """
8 |
9 | import numpy as np
10 | from model import RU_Net
11 | from train import Config
12 | from data_loader import ArbitraryDataLoader
13 |
14 | import os
15 | import SimpleITK as sitk
16 | import numpy as np
17 | import random
18 | import torch
19 | import torch.nn as nn
20 | from torch import optim
21 | import torch.nn.functional as F
22 | from torch.autograd import Variable
23 | import time
24 | from graphviz import Digraph
25 | from skimage.measure import label,regionprops
26 | from matplotlib import pyplot as pl
27 | from skimage import filters
28 | from skimage import data,util,transform
29 |
30 |
31 |
32 | def Predict(Model,ImageTensor,Shape,MaximumBbox):
33 | with torch.no_grad():
34 | PredSeg=Model.forward(ImageTensor)
35 | RegionOutput=np.zeros(ImageTensor.shape)
36 | RegionWeight=np.zeros(ImageTensor.shape)+0.001
37 | RoIs=PredSeg[2]
38 | #apply RoI predictions to a body-cropped large volume container
39 | #average predictions if RoIs are overlapped
40 | for i in range(len(PredSeg[0])):
41 | Coord=RoIs[i]*np.array([2,4,4,2,4,4])
42 | Weight=np.ones(np.asarray(PredSeg[0][i][0].shape))
43 | RegionOutput[0,:,Coord[0]:Coord[3],Coord[1]:Coord[4],Coord[2]:Coord[5]]+=PredSeg[0][i][0,1:].to('cpu').detach().numpy()
44 | RegionWeight[0,:,Coord[0]:Coord[3],Coord[1]:Coord[4],Coord[2]:Coord[5]]+=Weight[1:]
45 | RegionOutput/=RegionWeight
46 |
47 |
48 |
49 | return RegionOutput
50 |
51 | if __name__=='__main__':
52 |
53 | opt=[Config('RF64'),Config('RF88'),Config('RF112')]
54 | Models=[RU_Net(opt[0]).to(opt[0].GPU),RU_Net(opt[1]).to(opt[1].GPU),RU_Net(opt[2]).to(opt[2].GPU)]
55 | for mid,Model in enumerate(Models):
56 | Model.load_state_dict(torch.load(opt[mid].WEIGHT_PATH))
57 | Model.eval()
58 | Root='./Data/Test/'
59 | PatientNames=os.listdir(Root)
60 | PatientNames=sorted(PatientNames)
61 | NumPatients=len(PatientNames)
62 | for i in range(NumPatients):
63 | Patient=PatientNames[i]
64 | ImageTensor,Shape,MaximumBbox=ArbitraryDataLoader(Patient,opt[0],'Test')
65 | RegionOutput=np.zeros(ImageTensor.shape)
66 |
67 | time1=time.time()
68 | #Ensemble by averaging predictions
69 | for j in range(len(Models)):
70 | RegionOutput+=Predict(Models[j],ImageTensor,Shape,MaximumBbox)
71 | RegionOutput/=len(Models)
72 | print("time used: ",time.time()-time1)
73 |
74 | #body-cropped volume back to whole volume container
75 | OutputWhole=np.zeros(Shape,dtype=np.float)
76 | OutputWhole[MaximumBbox[0]:MaximumBbox[3],MaximumBbox[1]:MaximumBbox[4],MaximumBbox[2]:MaximumBbox[5]]=RegionOutput[0,0]
77 | #Back to ITK images for storage
78 | OutputWhole*=255
79 | OutputWhole=OutputWhole.astype(np.uint8)
80 | OutputWhole=sitk.GetImageFromArray(OutputWhole)
81 | OutputWhole.SetSpacing(opt[0].TO_SPACING)
82 | sitk.WriteImage(OutputWhole,'./Output/'+Patient+'/EnsemblePreds.mhd')
83 |
84 |
85 |
--------------------------------------------------------------------------------
/train.py:
--------------------------------------------------------------------------------
1 | #!/usr/bin/env python2
2 | # -*- coding: utf-8 -*-
3 | """
4 | Created on Mon Jun 11 15:10:18 2018
5 |
6 | @author: customer
7 | """
8 | import os
9 | import SimpleITK as sitk
10 | import numpy as np
11 | import random
12 | import torch
13 | from torch import optim
14 | import torch.nn.functional as F
15 | from torch.autograd import Variable
16 | import cv2
17 |
18 | from data_loader import DataLoader
19 | from model import RU_Net
20 | inplace=True
21 |
22 |
23 | STAGE_DILATIONS={'RF64':[1,1,1],'RF88':[1,1,2],'RF112':[1,2,2]}
24 | TAG='RF112'# or 'RF64' or 'RF88'
25 | class Config():
26 | def __init__(self,TAG):
27 | self.TAG=TAG
28 | self.STAGE_DILATION=STAGE_DILATIONS[TAG]
29 | self.DICT_CLASS={0:'Background', 1:'Cancer'}
30 | self.MAX_ROIS_TEST={'Background':0,'Cancer':10}
31 | self.MAX_ROIS_TRAIN={'Background':0,'Cancer':2}
32 | self.MAX_ROI_SIZE=[24,96,96]
33 | self.TO_SPACING=[1,1,4]
34 | self.DOWN_SAMPLE=[2,4,4]
35 | self.DATA_ROOT='./Data/'
36 | self.INPLACE=True
37 | self.GPU='cuda:1'
38 | self.MAX_EPOCHS=50
39 | self.WEIGHT_PATH='./Weights/'+self.TAG+'.pkl'
40 | self.TEST_ONLY=False
41 | self.BASE_CHANNELS=48
42 | opt=Config(TAG)
43 |
44 |
45 | def MultiClassDiceLossFunc(y_pred,y_true):
46 | overlap=torch.zeros([1]).cuda(opt.GPU)
47 | bottom=torch.zeros([1]).cuda(opt.GPU)
48 | for i in range(1,len(opt.DICT_CLASS.keys())):
49 | overlap+=torch.sum(y_pred[0,i]*y_true[0,i])
50 | bottom+=torch.sum(y_pred[0,i])+torch.sum(y_true[0,i])
51 | return 1-2*(overlap+1e-4)/(bottom+1e-4)
52 | def RoIDiceLossFunc(y_pred,y_true):
53 | overlap=torch.zeros([1]).cuda(opt.GPU)
54 | bottom=torch.zeros([1]).cuda(opt.GPU)
55 | for i in range(len(y_pred)):
56 | for j in range(1,len(opt.DICT_CLASS.keys())):
57 | overlap+=torch.sum(y_pred[i][0,j]*y_true[i][0,j])
58 | bottom+=torch.sum(y_pred[i][0,j])+torch.sum(y_true[i][0,j])
59 | return (1-2*overlap/bottom)
60 |
61 |
62 | def Predict(Patient,Subset):
63 | Image,LabelRegion,LabelContour,Shape,MaximumBbox=DataLoader(Patient,opt,Subset)
64 | Label=LabelRegion.to('cpu').detach().numpy()
65 |
66 | with torch.no_grad():
67 | PredSeg=Model.forward(Image)
68 | RegionOutput=np.zeros(Label.shape)
69 | RegionWeight=np.zeros(Label.shape)+0.001
70 | RoIs=PredSeg[2]
71 | #Apply RoI region predictions to in-body volume container
72 | #If overlapped, average
73 | for i in range(len(PredSeg[0])):
74 | Coord=RoIs[i]*np.array([2,4,4,2,4,4])
75 | Weight=np.ones(np.asarray(PredSeg[0][i][0].shape))
76 | RegionOutput[0,:,Coord[0]:Coord[3],Coord[1]:Coord[4],Coord[2]:Coord[5]]+=PredSeg[0][i][0]#.to('cpu').detach().numpy()
77 | RegionWeight[0,:,Coord[0]:Coord[3],Coord[1]:Coord[4],Coord[2]:Coord[5]]+=Weight
78 | RegionOutput/=RegionWeight
79 |
80 | #Apply RoI contour predictions to in-body volume container
81 | #If overlapped, average
82 | ContourOutput=np.zeros(Label.shape)
83 | ContourWeight=np.zeros(Label.shape)+0.001
84 | RoIs=PredSeg[2]
85 | for i in range(len(PredSeg[0])):
86 | Coord=RoIs[i]*np.array([2,4,4,2,4,4])
87 | Weight=np.ones(np.asarray(PredSeg[0][i][0].shape))
88 | ContourOutput[0,:,Coord[0]:Coord[3],Coord[1]:Coord[4],Coord[2]:Coord[5]]+=PredSeg[1][i][0]#.to('cpu').detach().numpy()
89 | ContourWeight[0,:,Coord[0]:Coord[3],Coord[1]:Coord[4],Coord[2]:Coord[5]]+=Weight
90 | ContourOutput/=ContourWeight
91 |
92 | #Apply in-body volume container to original volume size
93 | OutputWhole1=np.zeros(Shape,dtype=np.uint8)
94 | OutputWhole2=np.zeros(Shape,dtype=np.uint8)
95 | OutputWhole=np.zeros(Shape,dtype=np.uint8)
96 | OutputWhole1[MaximumBbox[0]:MaximumBbox[3],MaximumBbox[1]:MaximumBbox[4],MaximumBbox[2]:MaximumBbox[5]]=(RegionOutput[0,1]*255).astype(np.uint8)
97 | OutputWhole2[MaximumBbox[0]:MaximumBbox[3],MaximumBbox[1]:MaximumBbox[4],MaximumBbox[2]:MaximumBbox[5]]=(ContourOutput[0,1]*255).astype(np.uint8)
98 | #Save binary predictions
99 | OutputWhole[OutputWhole1>=128]=1
100 | OutputWhole[OutputWhole1<128]=0
101 | RegionOutput[RegionOutput>=0.5]=1
102 | RegionOutput[RegionOutput<0.5]=0
103 | Loss=1-2*np.sum(RegionOutput[0,1]*Label[0,1])/(np.sum(RegionOutput[0,1])+np.sum(Label[0,1]))
104 | OutputWhole1=sitk.GetImageFromArray(OutputWhole1)
105 | OutputWhole1.SetSpacing(opt.TO_SPACING)
106 | OutputWhole2=sitk.GetImageFromArray(OutputWhole2)
107 | OutputWhole2.SetSpacing(opt.TO_SPACING)
108 |
109 | #Draw bounding-boxes
110 | for Rid in range(len(RoIs)):
111 | color=(Rid+1,Rid+1,Rid+1)
112 |
113 | Coord=RoIs[Rid]*np.array([2,4,4,2,4,4])+np.array([MaximumBbox[0],MaximumBbox[1],MaximumBbox[2],MaximumBbox[0],MaximumBbox[1],MaximumBbox[2]])
114 | #Out-of-volume protection
115 | for protect in range(3):
116 | if Coord[protect+3]>=OutputWhole.shape[protect+0]:
117 | Coord[protect+3]=OutputWhole.shape[protect+0]-1
118 | #Draw rectangles
119 | Rgb=np.zeros([OutputWhole.shape[1],OutputWhole.shape[2],3],dtype=np.uint8)
120 | Rgb[:,:,0]=OutputWhole[Coord[0]]
121 | OutputWhole[Coord[0]]=cv2.rectangle(Rgb,(Coord[2],Coord[1]),(Coord[5],Coord[4]),color=color,thickness=2)[:,:,0]
122 | Rgb[:,:,0]=OutputWhole[Coord[3]]
123 | OutputWhole[Coord[3]]=cv2.rectangle(Rgb,(Coord[2],Coord[1]),(Coord[5],Coord[4]),color=color,thickness=2)[:,:,0]
124 |
125 | Rgb=np.zeros([OutputWhole.shape[0],OutputWhole.shape[1],3],dtype=np.uint8)
126 | Rgb[:,:,0]=OutputWhole[:,:,Coord[2]]
127 | OutputWhole[:,:,Coord[2]]=cv2.rectangle(Rgb,(Coord[1],Coord[0]),(Coord[4],Coord[3]),color=color,thickness=2)[:,:,0]
128 | Rgb[:,:,0]=OutputWhole[:,:,Coord[5]]
129 | OutputWhole[:,:,Coord[5]]=cv2.rectangle(Rgb,(Coord[1],Coord[0]),(Coord[4],Coord[3]),color=color,thickness=2)[:,:,0]
130 |
131 | Rgb=np.zeros([OutputWhole.shape[0],OutputWhole.shape[2],3],dtype=np.uint8)
132 | Rgb[:,:,0]=OutputWhole[:,Coord[1],:]
133 | OutputWhole[:,Coord[1],:]=cv2.rectangle(Rgb,(Coord[2],Coord[0]),(Coord[5],Coord[3]),color=color,thickness=2)[:,:,0]
134 | Rgb[:,:,0]=OutputWhole[:,Coord[4],:]
135 | OutputWhole[:,Coord[4],:]=cv2.rectangle(Rgb,(Coord[2],Coord[0]),(Coord[5],Coord[3]),color=color,thickness=2)[:,:,0]
136 | #Save mhds
137 | OutputWhole=sitk.GetImageFromArray(OutputWhole)
138 | OutputWhole.SetSpacing(opt.TO_SPACING)
139 | if os.path.exists('./Output/'+Patient)==False:
140 | os.makedirs('./Output/'+Patient)
141 | sitk.WriteImage(OutputWhole,'./Output/'+Patient+'/Pred_'+opt.TAG+'.mhd')
142 | sitk.WriteImage(OutputWhole1,'./Output/'+Patient+'/PredRegion_'+opt.TAG+'.mhd')
143 | sitk.WriteImage(OutputWhole2,'./Output/'+Patient+'/PredContour'+opt.TAG+'.mhd')
144 | return Loss,len(RoIs)
145 | def ToTensor(input):
146 | return 0
147 | if __name__=='__main__':
148 | lr=0.0001
149 | Model=RU_Net(opt)
150 | Model=Model.to(opt.GPU)
151 |
152 | optimizer1 = optim.Adam(list(Model.GlobalImageEncoder.parameters()),lr=lr,amsgrad=True)
153 |
154 | optimizer2 = optim.Adam(list(Model.GlobalImageEncoder.parameters())+\
155 | list(Model.LocalRegionDecoder.parameters()),lr=lr,amsgrad=True)
156 |
157 | TrainPatient=os.listdir(opt.DATA_ROOT+'Train')
158 | ValPatient=os.listdir(opt.DATA_ROOT+'Valid')
159 | TestPatient=os.listdir(opt.DATA_ROOT+'Test')
160 | NumTrain=len(TrainPatient)
161 | NumTest=len(TestPatient)
162 | NumVal=len(ValPatient)
163 |
164 | if not opt.TEST_ONLY:
165 | try:
166 | Model.load_state_dict(torch.load(opt.WEIGHT_PATH))
167 | print('Weights Loaded!')
168 | except:
169 | #Train Global Image Encoder and RoI locator
170 | for epoch in range(40):
171 | Model.train()
172 | for iteration in range(NumTrain):
173 | Model.train()#
174 | Patient=TrainPatient[random.randint(0,NumTrain-1)]
175 | Image,LabelRegion,LabelContour,Shape,MaximumBbox=DataLoader(Patient,opt,'Train')
176 | Label=LabelRegion
177 | optimizer1.zero_grad()
178 | PredSeg=Model.forward_RoI_Loc(Image,LabelRegion)#Model.train_forward(Image,LabelRegion,LabelContour,UseRoI=True)
179 | LossG=MultiClassDiceLossFunc(PredSeg[0],PredSeg[1])
180 | LossAll=LossG
181 | LossAll.backward()
182 | optimizer1.step()
183 | LossG=LossG.to('cpu').detach().numpy()
184 | print('loss={g=',LossG,'}')
185 | Loss=[]
186 | torch.save(Model.state_dict(), opt.WEIGHT_PATH)
187 |
188 | #Jointly train Global Image Encoder, RoI locator and Local Region Decoder
189 | Lowest=1
190 | for epoch in range(opt.MAX_EPOCHS):
191 | print('Epoch ',str(epoch),'/'+str(opt.MAX_EPOCHS))
192 | Model.train()#set_training(True)
193 | for iteration in range(NumTrain):
194 | Patient=TrainPatient[random.randint(0,NumTrain-1)]
195 | Image,LabelRegion,LabelContour,Shape,MaximumBbox=DataLoader(Patient,opt,'Train')
196 | optimizer2.zero_grad()
197 | PredSeg=Model.TrainForward(Image,LabelRegion,LabelContour)
198 | LossG=MultiClassDiceLossFunc(PredSeg[-1][0],PredSeg[-1][1])
199 | LossR=RoIDiceLossFunc(PredSeg[0],PredSeg[2])
200 | LossC=RoIDiceLossFunc(PredSeg[1],PredSeg[3])
201 | CWeight=1.0
202 | LossAll=LossG+LossR+CWeight*LossC
203 | LossAll.backward()
204 | optimizer2.step()
205 | LossG=LossG.to('cpu').detach().numpy()
206 | LossR=LossR.to('cpu').detach().numpy()
207 | LossC=LossC.to('cpu').detach().numpy()
208 | print('loss={g=',LossG,',r=',LossR,',c=',LossC,'}')
209 | Loss=[]
210 | Model.eval()#set_training(False)
211 |
212 | #Model selection according to Global Dice
213 | for iteration in range(NumVal):
214 | Patient=ValPatient[iteration]
215 | Loss_temp,NumRoIs=Predict(Patient,'Val')
216 | Loss+=[Loss_temp]
217 | print(Patient,' Loss=',Loss_temp)
218 | Loss=np.mean(np.array(Loss))
219 | if Loss