├── .github
└── workflows
│ └── tests.yml
├── .gradle
├── 7.5.1
│ ├── checksums
│ │ └── checksums.lock
│ ├── dependencies-accessors
│ │ ├── dependencies-accessors.lock
│ │ └── gc.properties
│ ├── executionHistory
│ │ ├── executionHistory.bin
│ │ └── executionHistory.lock
│ ├── fileChanges
│ │ └── last-build.bin
│ ├── fileHashes
│ │ ├── fileHashes.bin
│ │ ├── fileHashes.lock
│ │ └── resourceHashesCache.bin
│ └── gc.properties
├── buildOutputCleanup
│ ├── buildOutputCleanup.lock
│ ├── cache.properties
│ └── outputFiles.bin
├── file-system.probe
└── vcs-1
│ └── gc.properties
├── .idea
├── .gitignore
├── compiler.xml
├── gradle.xml
├── jarRepositories.xml
├── kotlinc.xml
├── misc.xml
├── uiDesigner.xml
└── vcs.xml
├── LICENSE
├── README.md
├── build.gradle.kts
├── build
├── classes
│ └── kotlin
│ │ ├── main
│ │ ├── META-INF
│ │ │ └── bamscope.kotlin_module
│ │ └── com
│ │ │ └── jslfree080
│ │ │ ├── bamscope
│ │ │ └── RunBAMScopeKt.class
│ │ │ ├── cli
│ │ │ ├── BAMScopeCommand$run$1.class
│ │ │ └── BAMScopeCommand.class
│ │ │ └── process
│ │ │ ├── BlockAdjustment.class
│ │ │ ├── CheckWidthAgain.class
│ │ │ ├── CheckWidthFirst.class
│ │ │ ├── ExtractReference$special$$inlined$sortedBy$1.class
│ │ │ ├── ExtractReference.class
│ │ │ ├── ParseRead.class
│ │ │ ├── PlotAlignment$letsPlot$p$1.class
│ │ │ ├── PlotAlignment$letsPlot$p$2.class
│ │ │ ├── PlotAlignment$letsPlot$p$3.class
│ │ │ ├── PlotAlignment$letsPlot$p$4.class
│ │ │ ├── PlotAlignment.class
│ │ │ ├── ReadSamtools.class
│ │ │ ├── RunSamtools.class
│ │ │ └── cigars
│ │ │ ├── CigarDeletion.class
│ │ │ ├── CigarInsertion.class
│ │ │ ├── CigarMatch.class
│ │ │ ├── CigarPadding.class
│ │ │ ├── CigarSkippedRegion.class
│ │ │ ├── CigarSoftClip.class
│ │ │ └── ParameterMID.class
│ │ └── test
│ │ ├── META-INF
│ │ └── bamscope.kotlin_module
│ │ └── com
│ │ └── jslfree080
│ │ └── process
│ │ ├── CheckWidthAgainTest.class
│ │ └── CheckWidthFirstTest.class
├── distributions
│ ├── bamscope-1.1.0.tar
│ └── bamscope-1.1.0.zip
├── kotlin
│ ├── bamscopejar-classes.txt
│ ├── compileKotlin
│ │ ├── cacheable
│ │ │ ├── caches-jvm
│ │ │ │ ├── inputs
│ │ │ │ │ ├── source-to-output.tab
│ │ │ │ │ ├── source-to-output.tab.keystream
│ │ │ │ │ ├── source-to-output.tab.keystream.len
│ │ │ │ │ ├── source-to-output.tab.len
│ │ │ │ │ ├── source-to-output.tab.values.at
│ │ │ │ │ ├── source-to-output.tab_i
│ │ │ │ │ └── source-to-output.tab_i.len
│ │ │ │ ├── jvm
│ │ │ │ │ └── kotlin
│ │ │ │ │ │ ├── class-attributes.tab
│ │ │ │ │ │ ├── class-attributes.tab.keystream
│ │ │ │ │ │ ├── class-attributes.tab.keystream.len
│ │ │ │ │ │ ├── class-attributes.tab.len
│ │ │ │ │ │ ├── class-attributes.tab.values.at
│ │ │ │ │ │ ├── class-attributes.tab_i
│ │ │ │ │ │ ├── class-attributes.tab_i.len
│ │ │ │ │ │ ├── class-fq-name-to-source.tab
│ │ │ │ │ │ ├── class-fq-name-to-source.tab.keystream
│ │ │ │ │ │ ├── class-fq-name-to-source.tab.keystream.len
│ │ │ │ │ │ ├── class-fq-name-to-source.tab.len
│ │ │ │ │ │ ├── class-fq-name-to-source.tab.values.at
│ │ │ │ │ │ ├── class-fq-name-to-source.tab_i
│ │ │ │ │ │ ├── class-fq-name-to-source.tab_i.len
│ │ │ │ │ │ ├── internal-name-to-source.tab
│ │ │ │ │ │ ├── internal-name-to-source.tab.keystream
│ │ │ │ │ │ ├── internal-name-to-source.tab.keystream.len
│ │ │ │ │ │ ├── internal-name-to-source.tab.len
│ │ │ │ │ │ ├── internal-name-to-source.tab.values.at
│ │ │ │ │ │ ├── internal-name-to-source.tab_i
│ │ │ │ │ │ ├── internal-name-to-source.tab_i.len
│ │ │ │ │ │ ├── package-parts.tab
│ │ │ │ │ │ ├── package-parts.tab.keystream
│ │ │ │ │ │ ├── package-parts.tab.keystream.len
│ │ │ │ │ │ ├── package-parts.tab.len
│ │ │ │ │ │ ├── package-parts.tab.values.at
│ │ │ │ │ │ ├── package-parts.tab_i
│ │ │ │ │ │ ├── package-parts.tab_i.len
│ │ │ │ │ │ ├── proto.tab
│ │ │ │ │ │ ├── proto.tab.keystream
│ │ │ │ │ │ ├── proto.tab.keystream.len
│ │ │ │ │ │ ├── proto.tab.len
│ │ │ │ │ │ ├── proto.tab.values.at
│ │ │ │ │ │ ├── proto.tab_i
│ │ │ │ │ │ ├── proto.tab_i.len
│ │ │ │ │ │ ├── source-to-classes.tab
│ │ │ │ │ │ ├── source-to-classes.tab.keystream
│ │ │ │ │ │ ├── source-to-classes.tab.keystream.len
│ │ │ │ │ │ ├── source-to-classes.tab.len
│ │ │ │ │ │ ├── source-to-classes.tab.values.at
│ │ │ │ │ │ ├── source-to-classes.tab_i
│ │ │ │ │ │ ├── source-to-classes.tab_i.len
│ │ │ │ │ │ ├── subtypes.tab
│ │ │ │ │ │ ├── subtypes.tab.keystream
│ │ │ │ │ │ ├── subtypes.tab.keystream.len
│ │ │ │ │ │ ├── subtypes.tab.len
│ │ │ │ │ │ ├── subtypes.tab.values.at
│ │ │ │ │ │ ├── subtypes.tab_i
│ │ │ │ │ │ ├── subtypes.tab_i.len
│ │ │ │ │ │ ├── supertypes.tab
│ │ │ │ │ │ ├── supertypes.tab.keystream
│ │ │ │ │ │ ├── supertypes.tab.keystream.len
│ │ │ │ │ │ ├── supertypes.tab.len
│ │ │ │ │ │ ├── supertypes.tab.values.at
│ │ │ │ │ │ ├── supertypes.tab_i
│ │ │ │ │ │ └── supertypes.tab_i.len
│ │ │ │ └── lookups
│ │ │ │ │ ├── counters.tab
│ │ │ │ │ ├── file-to-id.tab
│ │ │ │ │ ├── file-to-id.tab.keystream
│ │ │ │ │ ├── file-to-id.tab.keystream.len
│ │ │ │ │ ├── file-to-id.tab.len
│ │ │ │ │ ├── file-to-id.tab.values.at
│ │ │ │ │ ├── file-to-id.tab_i
│ │ │ │ │ ├── file-to-id.tab_i.len
│ │ │ │ │ ├── id-to-file.tab
│ │ │ │ │ ├── id-to-file.tab.keystream
│ │ │ │ │ ├── id-to-file.tab.keystream.len
│ │ │ │ │ ├── id-to-file.tab.len
│ │ │ │ │ ├── id-to-file.tab.values.at
│ │ │ │ │ ├── id-to-file.tab_i
│ │ │ │ │ ├── id-to-file.tab_i.len
│ │ │ │ │ ├── lookups.tab
│ │ │ │ │ ├── lookups.tab.keystream
│ │ │ │ │ ├── lookups.tab.keystream.len
│ │ │ │ │ ├── lookups.tab.len
│ │ │ │ │ ├── lookups.tab.values.at
│ │ │ │ │ ├── lookups.tab_i
│ │ │ │ │ └── lookups.tab_i.len
│ │ │ └── last-build.bin
│ │ └── local-state
│ │ │ └── build-history.bin
│ ├── compileTestKotlin
│ │ ├── cacheable
│ │ │ ├── caches-jvm
│ │ │ │ ├── inputs
│ │ │ │ │ ├── source-to-output.tab
│ │ │ │ │ ├── source-to-output.tab.keystream
│ │ │ │ │ ├── source-to-output.tab.keystream.len
│ │ │ │ │ ├── source-to-output.tab.len
│ │ │ │ │ ├── source-to-output.tab.values.at
│ │ │ │ │ ├── source-to-output.tab_i
│ │ │ │ │ └── source-to-output.tab_i.len
│ │ │ │ ├── jvm
│ │ │ │ │ └── kotlin
│ │ │ │ │ │ ├── class-attributes.tab
│ │ │ │ │ │ ├── class-attributes.tab.keystream
│ │ │ │ │ │ ├── class-attributes.tab.keystream.len
│ │ │ │ │ │ ├── class-attributes.tab.len
│ │ │ │ │ │ ├── class-attributes.tab.values.at
│ │ │ │ │ │ ├── class-attributes.tab_i
│ │ │ │ │ │ ├── class-attributes.tab_i.len
│ │ │ │ │ │ ├── class-fq-name-to-source.tab
│ │ │ │ │ │ ├── class-fq-name-to-source.tab.keystream
│ │ │ │ │ │ ├── class-fq-name-to-source.tab.keystream.len
│ │ │ │ │ │ ├── class-fq-name-to-source.tab.len
│ │ │ │ │ │ ├── class-fq-name-to-source.tab.values.at
│ │ │ │ │ │ ├── class-fq-name-to-source.tab_i
│ │ │ │ │ │ ├── class-fq-name-to-source.tab_i.len
│ │ │ │ │ │ ├── internal-name-to-source.tab
│ │ │ │ │ │ ├── internal-name-to-source.tab.keystream
│ │ │ │ │ │ ├── internal-name-to-source.tab.keystream.len
│ │ │ │ │ │ ├── internal-name-to-source.tab.len
│ │ │ │ │ │ ├── internal-name-to-source.tab.values.at
│ │ │ │ │ │ ├── internal-name-to-source.tab_i
│ │ │ │ │ │ ├── internal-name-to-source.tab_i.len
│ │ │ │ │ │ ├── proto.tab
│ │ │ │ │ │ ├── proto.tab.keystream
│ │ │ │ │ │ ├── proto.tab.keystream.len
│ │ │ │ │ │ ├── proto.tab.len
│ │ │ │ │ │ ├── proto.tab.values.at
│ │ │ │ │ │ ├── proto.tab_i
│ │ │ │ │ │ ├── proto.tab_i.len
│ │ │ │ │ │ ├── source-to-classes.tab
│ │ │ │ │ │ ├── source-to-classes.tab.keystream
│ │ │ │ │ │ ├── source-to-classes.tab.keystream.len
│ │ │ │ │ │ ├── source-to-classes.tab.len
│ │ │ │ │ │ ├── source-to-classes.tab.values.at
│ │ │ │ │ │ ├── source-to-classes.tab_i
│ │ │ │ │ │ └── source-to-classes.tab_i.len
│ │ │ │ └── lookups
│ │ │ │ │ ├── counters.tab
│ │ │ │ │ ├── file-to-id.tab
│ │ │ │ │ ├── file-to-id.tab.keystream
│ │ │ │ │ ├── file-to-id.tab.keystream.len
│ │ │ │ │ ├── file-to-id.tab.len
│ │ │ │ │ ├── file-to-id.tab.values.at
│ │ │ │ │ ├── file-to-id.tab_i
│ │ │ │ │ ├── file-to-id.tab_i.len
│ │ │ │ │ ├── id-to-file.tab
│ │ │ │ │ ├── id-to-file.tab.keystream
│ │ │ │ │ ├── id-to-file.tab.keystream.len
│ │ │ │ │ ├── id-to-file.tab.len
│ │ │ │ │ ├── id-to-file.tab.values.at
│ │ │ │ │ ├── id-to-file.tab_i
│ │ │ │ │ ├── id-to-file.tab_i.len
│ │ │ │ │ ├── lookups.tab
│ │ │ │ │ ├── lookups.tab.keystream
│ │ │ │ │ ├── lookups.tab.keystream.len
│ │ │ │ │ ├── lookups.tab.len
│ │ │ │ │ ├── lookups.tab.values.at
│ │ │ │ │ ├── lookups.tab_i
│ │ │ │ │ └── lookups.tab_i.len
│ │ │ └── last-build.bin
│ │ └── local-state
│ │ │ └── build-history.bin
│ ├── kaptGenerateStubsKotlin
│ │ ├── cacheable
│ │ │ ├── caches-jvm
│ │ │ │ ├── inputs
│ │ │ │ │ ├── source-to-output.tab
│ │ │ │ │ ├── source-to-output.tab.keystream
│ │ │ │ │ ├── source-to-output.tab.keystream.len
│ │ │ │ │ ├── source-to-output.tab.len
│ │ │ │ │ ├── source-to-output.tab.values.at
│ │ │ │ │ ├── source-to-output.tab_i
│ │ │ │ │ └── source-to-output.tab_i.len
│ │ │ │ ├── jvm
│ │ │ │ │ └── kotlin
│ │ │ │ │ │ ├── class-attributes.tab
│ │ │ │ │ │ ├── class-attributes.tab.keystream
│ │ │ │ │ │ ├── class-attributes.tab.keystream.len
│ │ │ │ │ │ ├── class-attributes.tab.len
│ │ │ │ │ │ ├── class-attributes.tab.values.at
│ │ │ │ │ │ ├── class-attributes.tab_i
│ │ │ │ │ │ ├── class-attributes.tab_i.len
│ │ │ │ │ │ ├── class-fq-name-to-source.tab
│ │ │ │ │ │ ├── class-fq-name-to-source.tab.keystream
│ │ │ │ │ │ ├── class-fq-name-to-source.tab.keystream.len
│ │ │ │ │ │ ├── class-fq-name-to-source.tab.len
│ │ │ │ │ │ ├── class-fq-name-to-source.tab.values.at
│ │ │ │ │ │ ├── class-fq-name-to-source.tab_i
│ │ │ │ │ │ ├── class-fq-name-to-source.tab_i.len
│ │ │ │ │ │ ├── internal-name-to-source.tab
│ │ │ │ │ │ ├── internal-name-to-source.tab.keystream
│ │ │ │ │ │ ├── internal-name-to-source.tab.keystream.len
│ │ │ │ │ │ ├── internal-name-to-source.tab.len
│ │ │ │ │ │ ├── internal-name-to-source.tab.values.at
│ │ │ │ │ │ ├── internal-name-to-source.tab_i
│ │ │ │ │ │ ├── internal-name-to-source.tab_i.len
│ │ │ │ │ │ ├── package-parts.tab
│ │ │ │ │ │ ├── package-parts.tab.keystream
│ │ │ │ │ │ ├── package-parts.tab.keystream.len
│ │ │ │ │ │ ├── package-parts.tab.len
│ │ │ │ │ │ ├── package-parts.tab.values.at
│ │ │ │ │ │ ├── package-parts.tab_i
│ │ │ │ │ │ ├── package-parts.tab_i.len
│ │ │ │ │ │ ├── proto.tab
│ │ │ │ │ │ ├── proto.tab.keystream
│ │ │ │ │ │ ├── proto.tab.keystream.len
│ │ │ │ │ │ ├── proto.tab.len
│ │ │ │ │ │ ├── proto.tab.values.at
│ │ │ │ │ │ ├── proto.tab_i
│ │ │ │ │ │ ├── proto.tab_i.len
│ │ │ │ │ │ ├── source-to-classes.tab
│ │ │ │ │ │ ├── source-to-classes.tab.keystream
│ │ │ │ │ │ ├── source-to-classes.tab.keystream.len
│ │ │ │ │ │ ├── source-to-classes.tab.len
│ │ │ │ │ │ ├── source-to-classes.tab.values.at
│ │ │ │ │ │ ├── source-to-classes.tab_i
│ │ │ │ │ │ ├── source-to-classes.tab_i.len
│ │ │ │ │ │ ├── subtypes.tab
│ │ │ │ │ │ ├── subtypes.tab.keystream
│ │ │ │ │ │ ├── subtypes.tab.keystream.len
│ │ │ │ │ │ ├── subtypes.tab.len
│ │ │ │ │ │ ├── subtypes.tab.values.at
│ │ │ │ │ │ ├── subtypes.tab_i
│ │ │ │ │ │ ├── subtypes.tab_i.len
│ │ │ │ │ │ ├── supertypes.tab
│ │ │ │ │ │ ├── supertypes.tab.keystream
│ │ │ │ │ │ ├── supertypes.tab.keystream.len
│ │ │ │ │ │ ├── supertypes.tab.len
│ │ │ │ │ │ ├── supertypes.tab.values.at
│ │ │ │ │ │ ├── supertypes.tab_i
│ │ │ │ │ │ └── supertypes.tab_i.len
│ │ │ │ └── lookups
│ │ │ │ │ ├── counters.tab
│ │ │ │ │ ├── file-to-id.tab
│ │ │ │ │ ├── file-to-id.tab.keystream
│ │ │ │ │ ├── file-to-id.tab.keystream.len
│ │ │ │ │ ├── file-to-id.tab.len
│ │ │ │ │ ├── file-to-id.tab.values.at
│ │ │ │ │ ├── file-to-id.tab_i
│ │ │ │ │ ├── file-to-id.tab_i.len
│ │ │ │ │ ├── id-to-file.tab
│ │ │ │ │ ├── id-to-file.tab.keystream
│ │ │ │ │ ├── id-to-file.tab.keystream.len
│ │ │ │ │ ├── id-to-file.tab.len
│ │ │ │ │ ├── id-to-file.tab.values.at
│ │ │ │ │ ├── id-to-file.tab_i
│ │ │ │ │ ├── id-to-file.tab_i.len
│ │ │ │ │ ├── lookups.tab
│ │ │ │ │ ├── lookups.tab.keystream
│ │ │ │ │ ├── lookups.tab.keystream.len
│ │ │ │ │ ├── lookups.tab.len
│ │ │ │ │ ├── lookups.tab.values.at
│ │ │ │ │ ├── lookups.tab_i
│ │ │ │ │ └── lookups.tab_i.len
│ │ │ └── last-build.bin
│ │ └── local-state
│ │ │ └── build-history.bin
│ └── kaptGenerateStubsTestKotlin
│ │ ├── cacheable
│ │ └── last-build.bin
│ │ └── local-state
│ │ └── build-history.bin
├── libs
│ └── bamscope.jar
├── reports
│ └── tests
│ │ └── test
│ │ ├── classes
│ │ ├── com.jslfree080.process.CheckWidthAgainTest.html
│ │ └── com.jslfree080.process.CheckWidthFirstTest.html
│ │ ├── css
│ │ ├── base-style.css
│ │ └── style.css
│ │ ├── index.html
│ │ ├── js
│ │ └── report.js
│ │ └── packages
│ │ └── com.jslfree080.process.html
├── scripts
│ ├── bamscope
│ └── bamscope.bat
├── test-results
│ └── test
│ │ ├── TEST-com.jslfree080.process.CheckWidthAgainTest.xml
│ │ ├── TEST-com.jslfree080.process.CheckWidthFirstTest.xml
│ │ └── binary
│ │ ├── output.bin
│ │ ├── output.bin.idx
│ │ └── results.bin
└── tmp
│ ├── jar
│ └── MANIFEST.MF
│ └── kapt3
│ ├── classes
│ └── main
│ │ └── META-INF
│ │ └── native-image
│ │ └── picocli-generated
│ │ └── com.jslfree080
│ │ └── bamscope
│ │ ├── proxy-config.json
│ │ ├── reflect-config.json
│ │ └── resource-config.json
│ ├── incApCache
│ ├── main
│ │ ├── ap-classpath-entries.bin
│ │ ├── apt-cache.bin
│ │ ├── classpath-entries.bin
│ │ ├── classpath-structure.bin
│ │ └── java-cache.bin
│ └── test
│ │ ├── ap-classpath-entries.bin
│ │ ├── classpath-entries.bin
│ │ └── classpath-structure.bin
│ ├── incrementalData
│ └── main
│ │ ├── META-INF
│ │ └── bamscope.kotlin_module
│ │ └── com
│ │ └── jslfree080
│ │ ├── bamscope
│ │ └── RunBAMScopeKt.class
│ │ ├── cli
│ │ └── BAMScopeCommand.class
│ │ └── process
│ │ ├── BlockAdjustment.class
│ │ ├── CheckWidthAgain.class
│ │ ├── CheckWidthFirst.class
│ │ ├── ExtractReference.class
│ │ ├── ParseRead.class
│ │ ├── PlotAlignment.class
│ │ ├── ReadSamtools.class
│ │ ├── RunSamtools.class
│ │ └── cigars
│ │ ├── CigarDeletion.class
│ │ ├── CigarInsertion.class
│ │ ├── CigarMatch.class
│ │ ├── CigarPadding.class
│ │ ├── CigarSkippedRegion.class
│ │ ├── CigarSoftClip.class
│ │ └── ParameterMID.class
│ └── stubs
│ └── main
│ ├── com
│ └── jslfree080
│ │ ├── bamscope
│ │ ├── RunBAMScopeKt.java
│ │ └── RunBAMScopeKt.kapt_metadata
│ │ ├── cli
│ │ ├── BAMScopeCommand.java
│ │ └── BAMScopeCommand.kapt_metadata
│ │ └── process
│ │ ├── BlockAdjustment.java
│ │ ├── BlockAdjustment.kapt_metadata
│ │ ├── CheckWidthAgain.java
│ │ ├── CheckWidthAgain.kapt_metadata
│ │ ├── CheckWidthFirst.java
│ │ ├── CheckWidthFirst.kapt_metadata
│ │ ├── ExtractReference.java
│ │ ├── ExtractReference.kapt_metadata
│ │ ├── ParseRead.java
│ │ ├── ParseRead.kapt_metadata
│ │ ├── PlotAlignment.java
│ │ ├── PlotAlignment.kapt_metadata
│ │ ├── ReadSamtools.java
│ │ ├── ReadSamtools.kapt_metadata
│ │ ├── RunSamtools.java
│ │ ├── RunSamtools.kapt_metadata
│ │ └── cigars
│ │ ├── CigarDeletion.java
│ │ ├── CigarDeletion.kapt_metadata
│ │ ├── CigarInsertion.java
│ │ ├── CigarInsertion.kapt_metadata
│ │ ├── CigarMatch.java
│ │ ├── CigarMatch.kapt_metadata
│ │ ├── CigarPadding.java
│ │ ├── CigarPadding.kapt_metadata
│ │ ├── CigarSkippedRegion.java
│ │ ├── CigarSkippedRegion.kapt_metadata
│ │ ├── CigarSoftClip.java
│ │ ├── CigarSoftClip.kapt_metadata
│ │ ├── ParameterMID.java
│ │ └── ParameterMID.kapt_metadata
│ └── error
│ └── NonExistentClass.java
├── gradle.properties
├── gradle
└── wrapper
│ ├── gradle-wrapper.jar
│ └── gradle-wrapper.properties
├── gradlew
├── gradlew.bat
├── settings.gradle.kts
└── src
├── main
└── kotlin
│ └── com
│ └── jslfree080
│ ├── bamscope
│ └── RunBAMScope.kt
│ ├── cli
│ └── CommandLine.kt
│ └── process
│ ├── BlockAdjustment.kt
│ ├── CheckWidthAgain.kt
│ ├── CheckWidthFirst.kt
│ ├── ExtractReference.kt
│ ├── ParseRead.kt
│ ├── PlotAlignment.kt
│ ├── ReadSamtools.kt
│ ├── RunSamtools.kt
│ └── cigars
│ ├── CigarDeletion.kt
│ ├── CigarInsertion.kt
│ ├── CigarMatch.kt
│ ├── CigarPadding.kt
│ ├── CigarSkippedRegion.kt
│ ├── CigarSoftClip.kt
│ └── ParameterMID.kt
└── test
└── kotlin
└── com
└── jslfree080
└── process
├── CheckWidthAgainTest.kt
└── CheckWidthFirstTest.kt
/.github/workflows/tests.yml:
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1 | name: Test
2 |
3 | on:
4 | push:
5 | branches:
6 | - main
7 |
8 | jobs:
9 | test:
10 | runs-on: ${{ matrix.os }}-latest
11 | strategy:
12 | matrix:
13 | java: [ 11 ]
14 | os: [ 'ubuntu', 'macos' ]
15 | steps:
16 | - uses: actions/checkout@v3
17 | - uses: actions/setup-java@v3
18 | with:
19 | java-version: ${{ matrix.Java }}
20 | distribution: 'adopt'
21 | cache: gradle
22 | - name: Grant execute permission for gradlew
23 | run: chmod +x gradlew
24 | - name: Test with Gradle
25 | run: ./gradlew --info test
26 | - name: Build the jar and run a commandline
27 | run: java -jar build/libs/bamscope.jar -h
28 |
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1 | #Mon Jan 23 03:13:41 KST 2023
2 | gradle.version=7.5.1
3 |
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/.idea/.gitignore:
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1 | # Default ignored files
2 | /shelf/
3 | /workspace.xml
4 |
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6 |
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/LICENSE:
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1 | MIT License
2 |
3 | Copyright (c) 2023 Jungsoo Lee
4 |
5 | Permission is hereby granted, free of charge, to any person obtaining a copy
6 | of this software and associated documentation files (the "Software"), to deal
7 | in the Software without restriction, including without limitation the rights
8 | to use, copy, modify, merge, publish, distribute, sublicense, and/or sell
9 | copies of the Software, and to permit persons to whom the Software is
10 | furnished to do so, subject to the following conditions:
11 |
12 | The above copyright notice and this permission notice shall be included in all
13 | copies or substantial portions of the Software.
14 |
15 | THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR
16 | IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY,
17 | FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE
18 | AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER
19 | LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM,
20 | OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE
21 | SOFTWARE.
22 |
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/README.md:
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1 | # bamscope
2 | 
3 | [](https://github.com/jslfree080/bamscope/blob/main/LICENSE)
4 |
5 | A command line tool (in Kotlin/JVM) for intuitively visualizing BAM alignments.
6 |
7 |
8 |
9 |
10 | ## Interpretation of Output Image
11 |
12 | The images produced display the alignment of the bases using colored blocks. The opacity of each block reflects the quality score of the base, with blocks valued near 1 displayed for high-quality scores near 40 and those valued near 0 for low-quality scores close to 0. The strandedness of each read's alignment is indicated in both the leftmost and rightmost columns, where '→' indicates a forward strand alignment and '←' indicates a reverse strand alignment.
13 |
14 | ## Prerequisites
15 |
16 | Bamscope requires **Java 11** or later versions to build and run.
17 |
18 | Also, [samtools](https://github.com/samtools/samtools) should be pre-installed to support accessing file formats such as BAM and FASTA.
19 |
20 | ## Building
21 |
22 | * Clone the repo:
23 | ```
24 | git clone https://github.com/jslfree080/bamscope.git
25 | cd bamscope/
26 | ```
27 |
28 | * Build an executable Bamscope jar with all dependencies packaged:
29 | ```
30 | ./gradlew build
31 | ```
32 |
33 | ## Usage
34 |
35 | ### Command line
36 |
37 | The resulting jar will be in `build/libs`.
38 |
39 | ```
40 | Usage: java -jar build/libs/bamscope.jar [options]
41 |
42 | Parameters:
43 | Input chromosomal position ex) (chr)N:XXXXXXXX
44 | or path to a VCF file. or path/to/x.vcf
45 | Path to an indexed BAM file. ex) path/to/x.bam
46 |
47 | Options:
48 | -f, --format= File format of an output image. default) png
49 | -h, --help Show this help message and exit.
50 | -o, --outPath= Path to an output image. default) .
51 | -r, --refPath= Path to a reference FASTA. ex) path/to/x.fasta(.fa)
52 | -s, --sPath= Path to the samtools. ex) /usr/local/bin/samtools
53 | -w, --width= Width of start to interest position. default) 50
54 | ```
55 |
56 | * Example usage for a specific position:
57 | ```
58 | java -jar build/libs/bamscope.jar 18:53254446 ~/Downloads/x.bam -r ~/Downloads/x.fasta -o ~/Desktop/
59 | ```
60 |
61 | * Example usage with VCF file (⚠️ This option may be computationally intensive):
62 | ```
63 | java -jar build/libs/bamscope.jar ~/Desktop/x.vcf ~/Downloads/x.bam -r ~/Downloads/x.fasta -o ~/Desktop/
64 | ```
65 |
66 | ## License
67 |
68 | Code and documentation released under the [MIT license](https://github.com/jslfree080/bamscope/blob/master/LICENSE).
69 |
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/build.gradle.kts:
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1 | /*
2 | * MIT License
3 | *
4 | * Copyright (c) 2023 Jungsoo Lee
5 | *
6 | * Permission is hereby granted, free of charge, to any person obtaining a copy
7 | * of this software and associated documentation files (the "Software"), to deal
8 | * in the Software without restriction, including without limitation the rights
9 | * to use, copy, modify, merge, publish, distribute, sublicense, and/or sell
10 | * copies of the Software, and to permit persons to whom the Software is
11 | * furnished to do so, subject to the following conditions:
12 | *
13 | * The above copyright notice and this permission notice shall be included in all
14 | * copies or substantial portions of the Software.
15 | *
16 | * THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR
17 | * IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY,
18 | * FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE
19 | * AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER
20 | * LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM,
21 | * OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE
22 | * SOFTWARE.
23 | */
24 |
25 | import org.jetbrains.kotlin.gradle.tasks.KotlinCompile
26 |
27 | plugins {
28 | kotlin("jvm") version "1.8.0"
29 | application
30 | kotlin("kapt") version "1.8.0" // kotlin.kapt
31 | }
32 |
33 | group = "com.jslfree080"
34 | version = "1.1.0"
35 |
36 | repositories {
37 | mavenCentral()
38 | }
39 |
40 | dependencies {
41 | testImplementation(kotlin("test"))
42 |
43 | implementation("info.picocli:picocli:4.7.0") // picocli library
44 | kapt("info.picocli:picocli-codegen:4.7.0") // picocli-codegen as kapt
45 |
46 | implementation("org.jetbrains.lets-plot:lets-plot-batik:3.0.0")
47 | implementation("org.jetbrains.lets-plot:lets-plot-jfx:3.0.0")
48 | implementation("org.jetbrains.lets-plot:lets-plot-common:3.0.0")
49 | implementation("org.jetbrains.lets-plot:lets-plot-kotlin-jvm:4.2.0")
50 | implementation("org.jetbrains.lets-plot:lets-plot-image-export:3.0.0")
51 | implementation("org.slf4j:slf4j-simple:2.0.6") // dependencies for lets-plot library
52 | }
53 |
54 | tasks.test {
55 | useJUnitPlatform()
56 | }
57 |
58 | tasks.withType {
59 | kotlinOptions.jvmTarget = "11" // version of jvmTarget
60 | }
61 |
62 | kapt { // kapt block with the argument project
63 | arguments {
64 | arg("project", "${project.group}/${project.name}")
65 | }
66 | }
67 |
68 | application {
69 | mainClass.set("RunBAMScopeKt")
70 | }
71 |
72 | tasks.withType {
73 | // Otherwise you'll get a "No main manifest attribute" error
74 | manifest {
75 | attributes["Main-Class"] = "com.jslfree080.bamscope.RunBAMScopeKt"
76 | archiveFileName.set("bamscope.jar")
77 | }
78 |
79 | // To avoid the duplicate handling strategy error
80 | duplicatesStrategy = DuplicatesStrategy.EXCLUDE
81 |
82 | // To add all of the dependencies
83 | from(sourceSets.main.get().output)
84 |
85 | dependsOn(configurations.runtimeClasspath)
86 | from({
87 | configurations.runtimeClasspath.get().filter { it.name.endsWith("jar") }.map { zipTree(it) }
88 | })
89 | }
90 |
91 | // ./gradlew build |or| ./gradlew clean build (Delete all the previous version of the dependencies)
92 | // java -jar build/libs/bamscope.jar 18:53253385 ~/Downloads/*.bam -r ~/Downloads/human_g1k_v37_decoy.fasta -o ~/Desktop/
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